http://csm.colostate-pueblo.edu/_layouts/feed.aspx?xsl=1&web=/&page=afffcef8-c31d-4f99-b147-f6786c4269a6&wp=06a5e05b-be75-40a4-8714-63006bccb4e660/Communications/EVENTS/Pages/eventdisplay.aspx?EventID=12<div class=ExternalClass31FC9A7C18C04A01A4798262E80348EF>Regulation of MCT1 Function By Intracellular pH and cAMP analogs in Cerebrovascular Endothelial Cells<br>Ms. Amy Uhernik, Graduate Student Colorado State University—Pueblo</div> <p>Monocarboxylic Acid Transporter 1 (MCT1) is expressed on the luminal and abluminal membranes of cerebrovascular endothelial cells where it is the sole transporter of monocarbox-ylic acids across the plasma membrane. Be-cause monocarboxylates, such as lactic acid, are important brain energy substrates and play important roles in brain pathologies, such as stroke, understanding factors that regulate MCT1 protein function is important for brain health and disease. In this study, we describe two distinctly different mechanisms of regulat-ing MCT1 in rat cerebrovascular endothelial cells. The first mechanism was changes in the intracellular pH which altered the driving force for lactate-proton co-transport. The second mechanism of regulating MCT1 was by treat-ment with membrane permeant cAMP analogs. Twenty minute exposure to 500 μM 8-Bromo-cAMP modulated MCT1 transport function by a mechanism that did not involve changes in cy-toplasmic pH. Together, these results suggest new modes of targeting M</p> <p>The Synthesis of Copper(I) Sensitive Fluorescent Compounds for Use in a Cellular System<br>Mr. Andrew Callaghan, Graduate Student Colorado State University—Pueblo</p> <p>Copper is an essential but toxic trace ele-ment in biological environments. It is know to be a factor in health and certain diseases. The ability to accurately detect and measure levels of copper in cellular systems would be benefi-cial in understanding normal and pathological aspects of copper. There has been limited work in the field of fluorescent copper sensors. Only a few of those are suitable for use in cel-lular imaging. The syntheses of some copper(I) sensitive fluorescent compounds are pre-sented. The compounds are designed to in-crease in fluorescence when bound to copper(I), which facilitates their use in biological im-aging. These compounds are based on a monoaza-tetrathia pseudo crown ethers and on the boron-dipyrrolemethene(BODIPY) fluorophore. The BODIPY core has many fa-vorable properties for use as a copper sensitive dye. The compounds presented contain modi-fications extending from this BODIPY core, thus changing the fluorescent properties as well as the physical properties to facilitate their use.</p> <p>Pizza, pop and goodies provided by the Dean’s Office</p> <div class=ExternalClass31FC9A7C18C04A01A4798262E80348EF></div>Chem 1062009-11-20 12:00:002009-11-20 13:00:00<div class=ExternalClass31FC9A7C18C04A01A4798262E80348EF>Regulation of MCT1 Function By Intracellular pH and cAMP analogs in Cerebrovascular Endothelial Cells<br>Ms. Amy Uhernik, Graduate Student Colorado State University—Pueblo</div> <p>Monocarboxylic Acid Transporter 1 (MCT1) is expressed on the luminal and abluminal membranes of cerebrovascular endothelial cells where it is the sole transporter of monocarbox-ylic acids across the plasma membrane. Be-cause monocarboxylates, such as lactic acid, are important brain energy substrates and play important roles in brain pathologies, such as stroke, understanding factors that regulate MCT1 protein function is important for brain health and disease. In this study, we describe two distinctly different mechanisms of regulat-ing MCT1 in rat cerebrovascular endothelial cells. The first mechanism was changes in the intracellular pH which altered the driving force for lactate-proton co-transport. The second mechanism of regulating MCT1 was by treat-ment with membrane permeant cAMP analogs. Twenty minute exposure to 500 μM 8-Bromo-cAMP modulated MCT1 transport function by a mechanism that did not involve changes in cy-toplasmic pH. Together, these results suggest new modes of targeting M</p> <p>The Synthesis of Copper(I) Sensitive Fluorescent Compounds for Use in a Cellular System<br>Mr. Andrew Callaghan, Graduate Student Colorado State University—Pueblo</p> <p>Copper is an essential but toxic trace ele-ment in biological environments. It is know to be a factor in health and certain diseases. The ability to accurately detect and measure levels of copper in cellular systems would be benefi-cial in understanding normal and pathological aspects of copper. There has been limited work in the field of fluorescent copper sensors. Only a few of those are suitable for use in cel-lular imaging. The syntheses of some copper(I) sensitive fluorescent compounds are pre-sented. The compounds are designed to in-crease in fluorescence when bound to copper(I), which facilitates their use in biological im-aging. These compounds are based on a monoaza-tetrathia pseudo crown ethers and on the boron-dipyrrolemethene(BODIPY) fluorophore. The BODIPY core has many fa-vorable properties for use as a copper sensitive dye. The compounds presented contain modi-fications extending from this BODIPY core, thus changing the fluorescent properties as well as the physical properties to facilitate their use.</p> <p>Pizza, pop and goodies provided by the Dean’s Office</p> <div class=ExternalClass31FC9A7C18C04A01A4798262E80348EF></div>/Communications/EVENTS/Pages/eventdisplay.aspx?EventID=13<div class=ExternalClass0E3EF28B9D104B3DA0E146C7A1152A74> <div>Tentative, more info coming soon.</div></div>Chem 1062009-12-04 12:00:002009-12-04 13:00:00<div class=ExternalClass0E3EF28B9D104B3DA0E146C7A1152A74> <div>Tentative, more info coming soon.</div></div>