Biotech Laboratory
Students from the Biotech Lab have won awards
in the several regional Tri Beta Meetings. This includes the sweep
of the 1998 conference at the Air Force Academy of the first two
places in both Undergraduate and Graduate Divisions.
Students have also won the Eli Lilly award at SACNAS (1998)
and the MAC undergraduate award at the American Society for Cell
Biology meeting (1999)
The Biotechnology Laboratory is set up for all basic and many
advanced application of Molecular and Cellular Biology all performed
by undergraduate students.
Facilities available allow undergraduate and graduates students as well as faculty to
use:
Molecular Biology techniques available and equipment for use
Equipment
- Refrigerated centrifuge [One clinical refrigerated centrifuge and one medium speed (Fisher 13K Marathon)
- Beckmann Ultra Centrifuge (in basement)
- Microfuges
- Micropipettes (including electronic and syringe multidispensing pipettes)
- Heating blocks
- UV/VIS Spectrophotometer (Genosys system)
- Hybridization oven for blots
- Thermocyclers for PCR
- Incubators (both walkin (37oC) and bench top (currently 30oC and 37oC)]
- Walk-in cold room (4oC)
- Shaking incubators (currently 30oC and 37oC)
- Shaking platforms
- Electrophoresis chambers for both Nucleic Acid and Protein Gels
- Transblotter for transfer of Proteins or Nucleic acids for blots
- Electroporators
- Vortexes
- Sonicator for cell disruption
- Refrigerators (4o), Frost Free (-20oC) Freezer, and (-80oC) Freezer
- Epi-Fluorescence microscope with digital imaging system (Zeiss Axioskop with cooled CCD Spot Camera)
- Yeast Genetics Disecting Microscope (with micromanipulator)
- Digital Gel Documentation system (Kodak 1D EDAS system)
- CO2 incubator and Clean Hood for Mammalian Cell Culture
- Flow cytometer (Epics PROFILE II)
Techniques that are routinely done in the laboratory
DNA, RNA, and Protein Isolation
Nucleic Acid and Protein Gel electrophoresis
DNA, RNA, and Protein Blots (Southerns, northerns, and westerns)
Epi-Fluorescence Microscopy
Enzyme assays
Polymerase Chain Reaction
UV/VIS spectrophotometer
Flow Cytometry
Bacterial and Fungal Culture Analysis
Mammalian Cell Culture (in the Cell Biology Teaching Laboratory)
Fluorescence Microscope
Several projects have used these facilities including
Development of yeast wtih gene deletions in
specific aminopeptidase genes, Cell cycle analysis, microarray analysis of gene regulation
Analysis of genomic differences in Frankia nodulating plants
Analysis of DNA damage due to psoralen exposure
DNA typing of Rainbow and Cut-throat trout
Isolation and Characterization of Acid Phosphatase from Penicillium
Isolation and detection of DNA of pathogens in water supplies
Flow Cytometer
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