Hi Canon City High School students and teachers (Ms. Tremble
and Mr. Laughlin)
Hope you all had fun iwith working on your DNA and checking
out the facilities here at CSU Pueblo.Below are the gels and the
genotypes I could determine. Sorry some didn't come out too well
but we did cut a few corners compared to the research methods
used in DNA labs. Feel free to contact me about this DNA fingerprint
experiment and anything else.
Cheers,
- Dan Caprioglio, Ph.D.
- Professor of Biology
- Colorado State University Pueblo
- 2200 Bonforte Blvd.
- Pueblo CO 81001
- 719-549-2027
- 719-549-2732 FAX
- dan.caprioglio@colostate-pueblo.edu
Gel 1
| Lane |
Sample |
Genotype |
| 1 |
20 |
+/- |
| 2 |
33 |
+/- |
| 3 |
18 |
+/+ |
| 4 |
Standard |
|
| 5 |
27 |
-/- |
| 6 |
13 |
+/+ weak |
| 7 |
35 |
+/- |
| 8 |
13 |
mess up |
|
 |
-
-
-
- Gel 2
-
-
| Lane |
Sample |
Genotype |
| 1 |
Standard |
|
| 2 |
22 |
no amplification |
| 3 |
16 |
+/- |
| 4 |
30 |
-/- |
| 5 |
1 |
+/- |
| 6 |
29 |
+/- |
| 7 |
32 |
+/+ |
| 8 |
2 |
+/- |
|
 |
-
-
-
- Gel 3
-
| Lane |
Sample |
Genotype |
| 1 |
Standard |
|
| 2 |
4 |
+/- |
| 3 |
34 |
+/+ |
| 4 |
19 |
+/- |
| 5 |
23 |
+/- |
| 6 |
14 |
+/- |
| 7 |
12 |
+/- |
| 8 |
7 |
no amplification |
|
 |
-
-
-
-
- Gel 4
-
-
-
| Top |
| Lane |
Sample |
Genotype |
| 1 |
11 |
no amplification |
| 2 |
Blank |
|
| 3 |
25 |
+/- |
| 4 |
24 |
+/- |
| 5 |
10 |
+/- |
| 6 |
15 |
+/+ |
| 7 |
3 |
+/+ |
| 8 |
21 |
+/- |
| 9 |
26 |
+/- |
| 10 |
Standard |
|
| Bottom |
| 1 |
Standard |
|
| 2 |
31 |
-/- |
| 3 |
28 |
+/- |
| 4 |
17 |
+/+ |
| 5 |
6 |
+/- |
|
 |